Weefselregeneratie in de pulpa

Endodontologen benadrukken keer op keer hoe uniek het pulpaweefsel is. Pulpa bestaat uit een streng bindweefsel, dat veel van de karakteristieken van embryonaal weefsel heeft behouden. Onderzoekers hebben in een diermodel geëxperimenteerd met technieken die het pulpaweefsel kunnen regenereren, met gebruikmaking van stamcellen. Het mogelijk succes van weefselregeneratie lijkt te worden bepaald door een goed herstel van de bloedtoevoer, in combinatie met de stamceltoevoeging.

Regenerative endodontics is an innovative treatment concept aiming to regenerate pulp, dentin and root structures. In the diseased or necrotic tooth, the limitation in vascular supply renders successful tissue regeneration/generation in a whole tooth challenging. The aim of this study is to evaluate the ability of vascularized tissue to develop within a pulpless tooth using tissue engineering techniques. A pulpless tooth chamber, filled with collagen I gel containing isolated rat dental pulp cells (DPC) and angiogenic growth factors, was placed into a hole created in the femoral cortex or into its own tooth socket, respectively. The gross, histological and biochemical characteristics of the de novo tissue were evaluated at 4 and 8 weeks post-transplantation. Tooth revascularization and tissue generation was observed only in the femur group, confirming the important role of vascular supply in tissue regeneration. The addition of cells and growth factors significantly promoted connective tissue production in the tooth chamber. Successful revascularization and tissue regeneration in this model demonstrate the importance of a direct vascular supply and the advantages of a stem cell approach.

Bron

Srisuwan T, Tilkorn DJ, Al-Benna S, Abberton K, Messer HH, Thompson EW. Revascularization and tissue regeneration of an empty root canal space is enhanced by a direct blood supply and stem cells. Dent Traumatol 2013; 29: 84-91. doi: 10.1111/j.1600-9657.2012.01136.x. Epub 2012 Apr 23.